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Model Characteristics - Model:
An orthotopic murine model of human spinal metastasis
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| Model Descriptor |
An orthotopic murine model of human spinal metastasis
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| Official Nomenclature |
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| Genotype |
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| Species |
Mouse (Mus musculus)
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| Strain |
Not specified
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| Is This a Tool Strain? |
No
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Developmental Stage
(applies only to Zebrafish) |
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| Experimental Design |
Spinal cord compression secondary to spinal metastases occurs in up to 14% of all cancer patients, with an incidence of 20,000 cases per year in the US. Among the malignancies that metastasize to the spine, lung cancer is one of the most aggressive, with a 1-year survival rate of ~ 22%. There is currently no reproducible animal model of human spinal metastasis that allows for laboratory study of the human disease. Consequently, the authors sought to develop an orthotopic model of spinal metastasis by using a human lung cancer cell line, and to correlate neurological decline with tumor growth. To establish a model of spinal metastasis, the authors used a transperitoneal surgical approach to implant PC-14 lung tumors into the L-3 vertebral body of athymic nude mice.
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| Phenotype |
Lung cancer xenografts grew in all animals undergoing functional evaluation (24 mice) according to a reliable and reproducible time course, with paraplegia occurring at a median interval of 30 days following tumor implantation. Importantly, the analysis defined 4 key milestones based on components of the validated semiquantitative Basso-Beattie-Bresnahan (BBB) scale; these were observed in all animals, were consistent, and correlated with histological progression of tumor. In conclusion, the authors have developed an orthotopic murine model of human spinal metastasis in which neurological decline reproducibly correlates with severity of tumor progression. Although developed for lung cancer, this model can be expanded to study other types of metastatic or primary spinal tumors. Ultimately, this will allow testing of targeted therapies against specific tumor types.
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| Website for add. info |
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| Breeding Notes |
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| Sex Distribution of the Phenotype |
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| Submitted by |
caMOD, Curator
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| Principal Investigator / Lab |
Rhines*, Laurence D.
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| Comment |
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| Model Availability: This model is available from |
| Strain |
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