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Model Characteristics - Model:
Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors in mice
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| Model Descriptor |
Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors in mice
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| Official Nomenclature |
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| Genotype |
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| Species |
Mouse (Mus musculus)
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| Strain |
Not specified
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| Is This a Tool Strain? |
Yes
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Developmental Stage
(applies only to Zebrafish) |
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| Experimental Design |
Transgenic mice are currently generated by pronuclear injection; however, this technique is still relatively inefficient, technically demanding, costly, and impractical in most other animal species. Another approach to transgenesis is to use retroviruses as gene delivery vehicles because they are able to stably integrate into the genome of cells. Lentiviruses are a class of retroviruses that cause chronic illnesses in the host organisms they infect. Among retroviruses, lentiviruses have the distinguishing property of being able to infect both dividing and nondividing cells, and this ability has led to their development as gene delivery vehicles. To determine whether, in contrast to oncoretroviruses, lentiviruses might be immune to developmental silencing, we used lentiviral-based vectors to generate transgenic mice and a href=http://cancermodels.nci.nih.gov/camod/ViewModelAction.do?unprotected_method=populateModelCharacteristics&aModelID=150066301 target="_blank" rats/a.
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| Phenotype |
The technique of transgenesis by lentiviral gene delivery to one-cell embryos overcomes many of the limitations of pronuclear injection. It is more efficient, less invasive to the embryos, more cost-effective, and technically less demanding. Delivering lentiviruses by co-incubation with denuded embryos obviates the need for micromanipulation and may be an easier option for many laboratories wanting to make transgenic animals. Furthermore, because the lentiviral delivery technique does not require visualization of the pronucleus, it has the potential to be extended to other animal species. For example, using this technique we have generated transgenic rats, a species into which the introduction of exogenous genes has so far been difficult and inefficient.
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| Website for add. info |
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| Breeding Notes |
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| Sex Distribution of the Phenotype |
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| Submitted by |
caMOD, Curator
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| Principal Investigator / Lab |
Baltimore*, David
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| Comment |
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| Model Availability: This model is available from |
| Strain |
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