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Model Characteristics - Model:
Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors in rats
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| Model Descriptor |
Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors in rats
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| Official Nomenclature |
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| Genotype |
SD-Tg(GFP)2BalRrrc
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| Species |
Rat (Rattus norvegicus)
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| Strain |
SD
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| Is This a Tool Strain? |
Yes
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Developmental Stage
(applies only to Zebrafish) |
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| Experimental Design |
Transgenic mice are currently generated by pronuclear injection; however, this technique is still relatively inefficient, technically demanding, costly, and impractical in most other animal species. Another approach to transgenesis is to use retroviruses as gene delivery vehicles because they are able to stably integrate into the genome of cells. Lentiviruses are a class of retroviruses that cause chronic illnesses in the host organisms they infect. Among retroviruses, lentiviruses have the distinguishing property of being able to infect both dividing and nondividing cells, and this ability has led to their development as gene delivery vehicles. To determine whether, in contrast to oncoretroviruses, lentiviruses might be immune to developmental silencing, we used lentiviral-based vectors to generate transgenic a href=http://cancermodels.nci.nih.gov/camod/ViewModelAction.do?unprotected_method=populateModelCharacteristics&aModelID=150066316 target="_blank" mice/a and rats.
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| Phenotype |
To determine whether exogenous genes introduced into single-cell embryos via lentiviral vectors could be expressed in mammals other than mice, the FUGW lentivirus was delivered to single-cell rat embryos by perivitelline injection. Introduction of exogenous genes into the germ line of rats has, so far, been difficult and inefficient. Of the 22 founder pups born, 13 (59.1%) carried one or more proviral insertions as determined by Southern blot analysis, and 9 (40.9%) expressed GFP at levels detectable by direct viewing of the skin under a fluorescent microscope. GFP-positive founders were crossed to wild-type animals, and F1 progeny rats carrying as few as one copy of the provirus expressed GFP as determined by direct viewing with a fluorescent microscope, showing that a GFP-expressing transgene is not silenced by transmission through the germ line.
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| Website for add. info |
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| Breeding Notes |
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| Sex Distribution of the Phenotype |
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| Submitted by |
caMOD, Curator
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| Principal Investigator / Lab |
Baltimore*, David
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| Comment |
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| Model Availability: This model is available from |
| Strain |
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