|
|
|
Model Characteristics - Model:
Metastatic growth of lung cancer cells is extremely reduced in Vitamin D receptor knockout mice
|
| Model Descriptor |
Metastatic growth of lung cancer cells is extremely reduced in Vitamin D receptor knockout mice
|
| Official Nomenclature |
|
| Genotype |
|
| Species |
Mouse (Mus musculus)
|
| Strain |
C57BL/6-mixed
|
| Is This a Tool Strain? |
No
|
Developmental Stage
(applies only to Zebrafish) |
|
| Experimental Design |
Lung metastatic neoplasms are the major cause of cancer mortality. Despite the progress of diagnostic techniques and improvements in surgical procedures, the prognosis of patients with lung cancer is generally poor, even in the early stages of cancer. Epidemiological studies indicate a positive correlation with the prevalence of cancers and low serum levels of Vitamin D metabolites. 1¿,25-Dihydroxyvitamin D3 [1¿,25(OH)2D3] is a potent inhibitor of cancer cell proliferation in vitro. There is, however, no report demonstrating that 1¿,25(OH)2D3 is operative in vivo to inhibit metastatic growth of cancer cells. To verify this possibility, we generated a stable transfectant of the Lewis lung carcinoma (LLC) cell expressing green fluorescent protein (GFP) and examined its metastatic activity in wild-type mice and Vitamin D receptor (VDR) knockout mice that exhibit no Vitamin D-dependent calcemic activity and extremely high serum levels of 1¿,25(OH)2D3 due to the overexpression of the 1¿-hydroxylase gene.
|
| Phenotype |
Here, we show that 1α,25(OH)2D3 inhibits metastatic growth of lung cancer cells in the defined animal model and may work as an intrinsic factor for prevention of metastasis in intact animals. These findings establish a critical role for 1α,25(OH)2D3 in lung metastatic neoplasms and provide a new model for metastasis of malignant cells.
|
| Website for add. info |
|
| Breeding Notes |
|
| Sex Distribution of the Phenotype |
|
| Submitted by |
caMOD, Curator
|
| Principal Investigator / Lab |
Okano*, Toshio
|
| Comment |
|
| |
| Model Availability: This model is available from |
| Strain |
Distributor |
Stock number |
|
| |
 Comments (Please login to submit new comments) |
|
|
