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Model Characteristics - Model:
Aerosol delivery of chemotherapy in an orthotopic model of lung cancer
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| Model Descriptor |
Aerosol delivery of chemotherapy in an orthotopic model of lung cancer
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| Official Nomenclature |
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| Genotype |
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| Species |
Mouse (Mus musculus)
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| Strain |
BALB/c
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| Is This a Tool Strain? |
No
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Developmental Stage
(applies only to Zebrafish) |
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| Experimental Design |
Regional chemotherapy has been proposed as a treatment modality in a number of situations in oncology in order to increase exposure of the tumor to the drug, while minimizing systemic side-effects. Administration of drugs directly to the lungs via inhalation allows regional drug delivery to the lungs and airways with smaller doses and fewer systemic effects. The aim of this study was to evaluate the effect on tumor growth of gemcitabine delivered by aerosol in an orthotopic model of lung carcinoma. Large cell carcinoma (NCI-H460) cells were implanted intrabronchially in 24 male BALB/c nude mice on day (d) 0. Aerosols were delivered once a week from d1 to d29 using an endotracheal sprayer. Altogether, 16 animals received gemcitabine at 8 (n=8) and 12 mg/kg (n=8), and eight received a vehicle aerosol.
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| Phenotype |
All animals in the vehicle group developed a large infiltrating carcinoma. Comparatively, four of 13 (31%) animals treated with gemcitabine had no visible tumor and nine of 13 (69%) had a smaller carcinoma with a mean+/-SEM largest tumor diameter of 2.05+/-0.7 versus 5+/-0.3 mm in the vehicle group. Gemcitabine was well tolerated at 8 mg/kg. At 12 mg/kg, three cases of fatal pulmonary edema were observed, prompting a dose reduction to 8 mg/kg in the remaining animals. A dose effect was observed, with more marked tumor growth inhibition in the animals treated at 12 mg/kg on d1 and d8. In conclusion, in this study, an animal model of aerosolized chemotherapy in lung cancer was developed and demonstrated inhibition of orthotopic tumor growth by aerosol delivery of gemcitabine.
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| Website for add. info |
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| Breeding Notes |
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| Sex Distribution of the Phenotype |
Male Only
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| Submitted by |
caMOD, Curator
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| Principal Investigator / Lab |
Urban*, T
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| Comment |
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| Model Availability: This model is available from |
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